Author(s): Bharti G. Jadhav*, Amruta M. Jadhav, Abhay R. Shirode, Vilasrao J. Kadam
Preparative High Performance Liquid Chromatography (Prep. HPLC) is an important quantitative technique which is generally used for the isolation and purification of variety of chemicals including pharmaceutical compounds, natural products and biological samples. It is the most versatile and safest chromatographic technique which is used for the quality control of drug components. The attempt was to review different aspects of preparative HPLC. In this article, preparative HPLC is discussed with respect to instrumentation in comparison with analytical HPLC, hyphenation with other techniques, reported studies, applications and uses.