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Author(s): V. Asha Ranjani, T.Prabhakar, Girija Sankar

A simple and selective ,stability indicating LC method is described for the determination of Nicoumalone tablet dosage forms. The present paper deals with development and validation of a stability indicating reverse phase HPLC method for the estimation of Nicoumalone using Thermo BDS Hypersil column (250 mm X 4.6 mm, 5 µm). A mobile phase consisting of Acetonitrile: Ammonium Acetate buffer 0.01M, pH 6 adjusted using 0.1 N NaOH in 80:20 v/v ratio was employed in this study. The flow rate was set at 0.8 ml/min. Separation was performed at ambient temperature. Stability studies represented the degradation of drug in different condtions of acidic,alkali,oxidative and thermal. Linearity was observed in the range 48-112µg /ml for Nicoumalone (r2 =0.9986) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim.

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