News

Articles related to COVID-19 will be published in special issue.

Articles Statistics
48.6% 14.5%
Pharmaceutics
Manuscript Statistics
12.5% 21.9% 46.9%
Withdrawn

A VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PHENYLEPHRINE AND KETOROLAC IN INJECTABLE PREPARATIONS


Abstract

Author(s): P. J. Prasuna Sundari, Anitha kusuma, Prathima Srinivas

Omidria is an ophthalmic injectable of phenylephrine and ketorolac intended for use in cataract surgeries and intraocular lens replacement procedures. Objective of the present study was to develop and validate a specific, accurate, precise RP-HPLC method for the simultaneous estimation of phenylephrine and ketorolac in omidria. The proposed RP- HPLC method utilizes Std BDS C8column (250 mm × 4.6 mm id, 5μm particle size), mobile phase consisting of buffer and acetonitrile in the ratio of (30:70) and a flow rate of 1 ml/min. Quantitation was achieved with PDA detection at 220 nm. Separation was achieved at retention times of 2.313min and 3.090min for phenylephrine and ketorolac respectively. The method was validated as per ICH guidelines for accuracy, precision, linearity, limit of detection, limit of Quantitation and robustness. Limit of detection and limit of Quantitation were determined to be 0.091ppm 0.087ppm; 0.278ppm 0.021ppm for phenylephrine and ketorolac respectively. Linearity was achieved at concentration range of 20-120μg/ml and 6-36μg/ml for phenylephrine and ketorolac. It can be concluded from this study, that the developed RP-HPLC method may be applied in regular analysis for simultaneous estimation of phenylephrine and ketorolac in various dosage formulations.

Indexed in

Google Scholar

Chemical Abstract Services (CAS)

Science Central

Index Copernicus

Pharmaceutical Science Open Access Resources (PSOAR)

Rubriq

Directory of Research Journals Indexing

Journal Seeker

Directory of Science

Indian Science.in

Jour Informatics and China Knowledge Research Integrated (CNKI)

EBSCO

Publication Statistics
Vol... Vol... Vol... Vol... Vol... Vol... 0 5 10 15 20 Issues No.of Articles
6
World wide visitors
pharmaceutical analysis, international journal, chemical and pharmaceutical, journal of chemical, online open access journals, open access journals
Google Scholar citation report
Citations : 166

International Journal of Chemical and Pharmaceutical Analysis received 166 citations as per google scholar report

International Journal of Chemical and Pharmaceutical Analysis peer review process verified at publons

Indexed In