Cancer is a serious problem with significant impacts on health systems. Combination therapy is the cornerstone of cancer treatment today. Our research aims to obtain specific data about the agents that can be used in combination with chemotherapeutics in cancer treatment.
In our study MTT cytotoxicity assessment, transmission electron microscopic analysis of ultrastructural changes, detection of phosphatidylserine translocation and caspase 3/7 analysis were performed on Ishikawa cancer cell lines.
Cytotoxicity test results showed that resveratrol has ant proliferative activity on Ishikawa cells in a dose- dependent manner. Transmission electron microscopy findings indicated important ultra-structural changes on Ishikawa cells caused by resveratrol. Apoptotic profiles of Ishikawa cells were investigated via the annexin-V technique. Compared to the control Ishikawa cells in the population of resveratrol treated cells, the percentage of total apoptotic cells were detected to be 27.75%. Activation of caspases is an indicator of apoptosis pathways that are triggered into the cells. The total percentage of activated caspases was detected to be 5.35% when compared to that of control cells.
The highlight of our research is the transmission electron microscope examination. With this examination, morphological changes in mitochondria caused by the anticancer effect of resveratrol were determined.