Author(s): Sekar Ganesan, Senthil Kumar Natesan
Objective: To investigate in vitro and in vivo anti diabetic activity of Ensete superbum (E. superbum) fractions on high fat diet (HFD) with low dose Streptozotocin (STZ) induced type -2 diabetes mellitus in rats. Methods: Ensete superbum were successively extracted and then fractionated by Ethyl acetate and n-butanol. The in vitro antidiabetic activity of α-amylase and α- glucosidase was used to evaluate the potential activity of the fractions. The in vivo antidiabetic activity is evaluated against HFD/STZ induced type- 2 diabetic in rats at dose of 100mg/kg, 200mg/kg and 400mg/kg by mouth for 28 days. Results: Ethyl acetate fraction of Ensete superbum (EAFES) showed the highest antidiabetic activity and IC50 value of α-amylase inhibition (117.2±1.43) and α- glucosidase inhibition (105.80±1.65) than other fraction. In HFD/STZ (40mg/kg) diabetic rats the EAFES showed dose dependent significant hypoglycaemic property from body weight, blood glucose, serum lipids, serum cholesterol, serum triglycerides, urea, creatinine, hepatic enzymes and liver glycogen levels. The EAFES significantly (P < 0.01) increase the level of serum insulin. Histologically, focal necrosis was observed in diabetic rat pancreas; however, was less obvious in treated groups. Conclusion: The EAFES is a potent hypoglycaemic agent and beneficial in reducing the elevated blood glucose level, improve the lipid profile and insulin level and histopathological changes in pancreas of HFD/STZ?induced non?genetic rat model of type- 2 diabetes mellitus.