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Author(s): Ketaki Sharad Potdar1, Mallinath Shankareppa Kalshetti 2, Ravikant Yashwantrao Patil

RP-HPLC method have been developed for the simultaneous estimation of Alogliptin Benzoate and Pioglitazone HCl in pharmaceutical dosage form. RP-HPLC method was carried on Phenomenex C-18 column (150 mm × 4.6 mm, 5 μm) with Phenomenex Security Guard Cartridges C18(4x3mm) using a mobile phase acetonitrile: methanol: water: (30:22:48) as a mobile phase at 1.0ml/min flow rate at 268 nm. The linearity is found in the range of 10-50 μg/ml and 12-60 μg/ml with regression coefficient (r2= 0.998, and r2=0.997) for Alogliptin Benzoate and Pioglitazone HCl respectively. Alogliptin Benzoate and Pioglitazone hydrochloride have eluted at 4.6 and 6.5 mints respectively. This method is accurate and precise and can be employed for routine analysis of Alogliptin benzoate and Pioglitazone hydrochloride in different pharmaceutical dosage forms.

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