Author(s): Avdhoot Rameshwar Malokar, Ram Lokhande, Ravi Yadav
Most of the analytical method required, fast and reliable analytical technique as per current requirement of pharmaceutical industry. Hence a simple rugged and cost effective method was developed and validated for Metaxalone. By using this HPLC method, the time for analysis is reduced sensationally as compared to other method published in most of the literatures published. The method was set on Ascentis express C8 column (10cm x 4.0mm, 2.7µ) using 0.1% Triethylamine as a buffer (pH 3.0 with per chloric acid) and acetonitrile as organic modifier. The flow rate was set at 1.0ml\min and analysis time was 4 minutes with gradient elution. The detection was conducted at 210nm. The method was validated as per International Conference of Harmonization (ICH) Guidelines in terms of Specificity linearity and range, precision and robustness. Sample and standard concentration of metaxalone was 0.5mg/ml. linearity of standard was covered from 0.4mg/ml to 0.6mg/ml.