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Author(s): Prajakta Uttam Chate , Mallinath Shankareppa Kalshetti, Ravikant Yashwantrao Patil

RP-HPLC method have been developed for the simultaneous estimation of Valsartan and Nebivolol in pharmaceutical dosage form. RP-HPLC method was carried on Phenomenex C-18 column (150 mm × 4.6 mm, 5 ?) with Phenomenex Security Guard Cartridges C18(4x3mm) using a mobile phase acetonitrile:methanol:phosphate buffer with pH 3.0 (40:30:30) as a mobile phase at 1.0ml/min flow rate at 228 nm. The linearity was found to be in the range of 32-160 μg/ml and 2-10 μg/ml with regression coefficient (r2= 0.980, and r2=0.983) for Valsartan and Nebivolol respectively. The peaks obtained were sharp having clear baseline separation with a retention time 3.9min and 2.2 min for Valsartan and Nebivolol. This method is accurate and precise and can be employed for routine analysis of valsartan and nebivolol in different pharmaceutical dosage forms.

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