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Author(s): Sreelakshmi. Ma, R. L. C. Sasidharaa, Raviteja. B

An isocratic reversed-phase liquid chromatograpic assay method was developed for the quantitative determination of oxycodone and naltrexone. A Inetsil C-18, 5 μm column with a mobile phase containing Acetonitrile: Methanol: 0.1%Triethylamine buffer (pH-adjusted to 3 using o-phosphoric acid) 25:35:40(v/v). The flow rate was 1.0 mL/min and effluents were monitored at 250 nm. The retention times of oxycodone and naltrexone were 5.57 min and 2.60min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, and robustness.

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Citations : 440

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