Author(s): Zeba Mukhtar Aalam Farash, Amit Suryakant Tapkir, Praveen Chaudhari
Piceatannol is a stilbenoid and an important phytomolecule present in Cyperus rotundus Linn. plant. The major activity concerned is antiobesity activity of piceatannol. Compared with the analytical methods published in literatures, all methods have their drawbacks. Capillary electrophoresis needs a more expensive and less available instrument as compared with HPLC, even though only a tiny amount of sample is required for analysis. For GC analysis, stilbenoids need to be derivatized to enhance their volatility. In addition to the inherent limitations of instruments, some methods involve rather complicated extraction steps. Thus, a small quantity of Piceatannol presented in plants may be lost during the extraction, derivatization, or other sample preparation procedures. Hence, a method with fewer sample preparation steps is certainly preferred. There is no literature reported for the analysis of piceatannol in Cyperus rotundus linn. extract by HPLC, so there is need to establish a simple, sensitive, reliable, rapid and validated HPLC method for estimation and quantification of Piceatannol in C. rotundus Linn. extract. Following method provides a very reliable and accurate way for the quantification of piceatannol in Cyperus rotundus extract. The method was developed and validated on a C18 column with a gradient program using buffer and acetonitrile (ACN) as a mobile phase system. A good linear relationship between the concentrations (20–150%) and peak areas were obtained with the correlation coefficient (r2) of 0.9991.