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Author(s): Raghuram Reddy Adidala, Shravan Kumar Medi, Mounika Arrabelli2

Cancer refers to a group of illnesses that result from cells in the body growing abnormally. These cells divide and produce new cells in an uncontrolled way that can spread throughout the body and can cause damage to essential organs. The main aim of my work is to perform the simultaneous estimation and method development of Pemetrexed and folic acid in combination by using UFLC with PDA detector. UFLC Chromatograph was performed with an (Shimadzu LC-20AD Pump Photo Diode Array Detector) analytical Kromasil C18 column using Phosphate buffer: Acetonitrile: Methanol (75:15:10) as the mobile phase. The flow rate was set at 1 mL/min and the injection volume was 20 µL. The minimally acceptable correlation coefficient (r2 ) for the calibration curve was 0.99 and 0.992for Pemetrexed and folic acid respectively. Retention time of Pemetrexed and folic acid were 7.9 min and 3.6 min. respectively. The intra- and inter- day accuracies (% deviation) were within < ±10% for the QC samples

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pharmaceutical analysis, international journal, chemical and pharmaceutical, journal of chemical, online open access journals, open access journals
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Citations : 440

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