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DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR THE ESTIMATION OF HIBIFOLIN ISOLATED FROM THE ETHANOLIC EXTRACT OF MELOCHIA CORCHORIFOLIA LEAVES


Abstract

Author(s): Lekhana A.R., Palaksha M. N., Likhitha T. G.

Simple, precise and accurate zero orders derivative spectroscopic method has been developed and validated for the estimation of Hibifolin. The drug shows maximum absorption (λ max) at 664nm in Methanol solution and obeys Beer’s law in the concentration range of 50-250µg/ml. The linearity study carried and regression coefficient was found to be 0.9997 and it has showed good linearity, precision in this concentration range. The LOD and LOQ were found to be 2.6332 and 7.9014µg/ml. The % relative standard deviations were found less than 2. The method has been validated according to ICH guidelines for linearity, precision, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for reliable quantification of Hibifolin.

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