Author(s): Shirish Janardan Velankar, Ram Lokhande, Ravi Yadav, Ramchandra Pawar
A simple rugged and user-friendly and cost effective method to quantify low-molecular-weight formaldehyde and acetaldehyde in the food contact plastic, with 2, 4-Dinitrophenyl hydrazine (2, 4-DNPH) is presented using High Pressure Chromatography (HPLC). Use of 2, 4-DNPH ensure that the probable carbonyl compounds in the food contact plastics, can be monitored using the same method. The separation was achieved with Ascentis Express C18 (100 mm x 4.6 mm x 2.7µ) using Water: Acetonitrile (70: 30 v/v) with a flow rate of 2.0 ml /min. The Method was validated for the analytical parameters viz., Specificity, Limit of Detection (LOD), Limit of Quantitation (LOQ), Linearity and Range, Precision – System, Method and Spiked and Accuracy. For Bottle Gourd, the limit of detection and quantitation was found to be 10 and 30 PPM for formaldehyde with respect to (w.r.t.) sample concentration 10 mg/ ml. The limit of detection and quantitation was found to be 20 PPM and 60 PPM for acetaldehyde w.r.t sample concentration 10 mg/ ml. Primary contact plastic: The limit of detection and quantitation was found to be 250 and 750 PPM for formaldehyde w.r.t sample concentration 0.4 mg/ ml. The limit of detection and quantitation was found to be 500 PPM and 1500 PPM for acetaldehyde w.r.t sample concentration 0.4 mg/ ml.