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Author(s): Alemu Tadesse, Ariaya Hymete , Adnan A, Bekhit, Asrat Hailu

Objective: The aim of this study was to investigate the antileishmanial effect of the aqueous and methanolic extracts of Wushwush green tea samples against Leishmania major promastigotes. Methods: After evaporation and freeze-drying the extracts were dissolved in Dimethyl Sulfoxide (DMSO) 1% in PBS. The stock solutions of 128 mg/ml were prepared for the two solvents by dissolving 100 g of Wushwush green tea extract in culture media for anti-leishmanial assays. Clinical isolate of L. aethiopica strain (CL014/09) promastigotes were cultured in RPMI-1640 supplemented with 10% heat inactivated fetal calf serum (HIFCS), 1% L-glutamine and 1% penicilline-streptomycin solution. Results: Aqueous and methanolic extracts of Wushwush green tea showed significant leishmanicidal activity against L. major promastigotes. Conclusion: Our study revealed a significant pharmacological activity against promastigotes of L. major and suggests that green tea extract has the potential of being used in leishmaniasis treatment in combination with the available antileishmanial drugs

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pharmaceutical analysis, international journal, chemical and pharmaceutical, journal of chemical, online open access journals, open access journals
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Citations : 440

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