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Author(s): Badhe Manisha, K. Kamalakannan, Chaudhari Yogesh, Harshal Pawar

Ethyl acetate extract of strain NIO MU 19.2 showed significant antimicrobial activity against human test pathogens as well as multi-drug resistant pathogens from thirty-eight marine bacteria isolated from three sponges and three ascidians from Mumbai (India). A strain NIO MU 19.2 was subjected to morphological and biochemical characterization. In addition, fermentation media, modified media contents and fermentation conditions were also optimized for maximum production of antimicrobial metabolite. NIO MU 19.2 was mass cultured using optimized conditions and ethyl extract of broth was further fractionated with column chromatography. Through bioassay-guided fractionation, active fraction was identified and subjected for chemical studies such as HPLC, UV, IR, and NMR. Further studies are in progress in order to elucidate the structure and mode of action of bioactive molecule

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pharmaceutical analysis, international journal, chemical and pharmaceutical, journal of chemical, online open access journals, open access journals
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Citations : 440

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