Author(s): Swati D. Bakshi, Deepali M. Gangrade, Amit R. Sharma
To describe the relationship between the genome and phenotype in cells and organisms, increasing efforts are being made in the post-genomic era. It is clear that even a complete understanding of the state of genes, messages and proteins in a living organism does not reveal its phenotype. Therefore, researchers developed the metabolomics study in which metabolomes or the metabolomic complement of functional genomics is studied. Terms such metabolomics, metabolome, metabolites, metabonomics, etc. are mentioned in this text. An overview of target analysis, metabolic fingerprinting and metabolic foot printing is given. The methods for sample preparation and detection are also mentioned in this text. In sample preparation, methods such as quenching, extraction and sample concentration are studied. Also, the various analytical techniques or methodologies used in the metabolomics detection are described. A general description of these techniques i.e. mass spectrometry and NMR spectroscopy is given. The advantages and limitations of these techniques are highlighted.