Abstract
Author(s): Vaughan Charles Fernandes*1, E.V.S. Subrahmanyam2, Paramita Das3
The proposed method involves zero order derivative spectroscopy method. A novel, simple and rapid UV Spectrophotometric
determination method for simultaneous estimation of Metformin hydrochloride (MET) and Saxagliptin (SAXA) was successfully
developed and validated in bulk and pharmaceutical formulation. In this current work simultaneous estimation of MET and SAXA in
distilled water was performed by using UV spectroscopy. The zero-order derivative spectroscopy revealed that MET had a λmax of
237nm in distilled water and SAXA had λmax of 210 nm respectively. The linearity was found in the range of 2-16μg/ml for MET
and 5-50μg/ml for SAXA. The R2 value was found to be 0.9983 and slope y=0.002 for MET and R2 =0.9949 and slope y=0.064 for
SAXA
