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Author(s): Vaughan Fernandes*, E.V. S. Subrahmanyam, A R Shabaraya

A simple, accurate and precise spectrophotometric method has been developed for simultaneous determination of Saxagliptin (SAXA) and Metformin hydrochloride (MET) in a laboratory mixture. In absorbance ratio method, isobestic point is observed at 227 nm. Isobestic point (227nm) is considered as λ2 and absorbance maxima of Saxagliptin (210nm) is considered as λ1. Saxagliptin and Metformin hydrochloride were quantified using principle that absorbance difference between two points on mixture spectra is directly proportional to concentration of components of interest and independent of interfering component. All dilutions were prepared in distilled water. Linearity range was observed in the concentration range of solution 5?50 μg/ml for Saxagliptin and 2-16 μg/ml for Metformin hydrochloride. The methods were validated statistically and recovery study was performed to confirm the accuracy of both drugs.

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Citations : 440

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