The aim of the present work was to develop and validate a sensitive, simple, accurate, precise, and cost-effective UV
spectrophotometric method for the analysis cholinesterase inhibitor i.e Donepezil hydrochloride. The different analytical
performance parameters such as linearity, range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ)
were determined according to International Conference on Harmonization (ICH) Q2 (R1) guidelines. The study was performed in
Acetonitrile, water, and methanol. The peak (λmax) of Donepezil hydrochloride appeared at a wavelength of 231 nm. Beer-
Lambert’s law was obeyed in the concentration range of 4–20 μg/ml with correlation coefficient (R2) 0.999. The results of the study
demonstrated that the developed procedure is accurate, precise, and reproducible (relative standard deviation 2%), while being
simple, cheap and less time consuming. Therefore, this method can be suitably applied for the estimation of Donepezil
hydrochloride in prepared pharmaceutical formulations.

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