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RP-HPLC Method Development and Validation for Betahistine Hydrochloride Controlled Release Tablets


Author(s): Deepali M. Gangrade, Swati D. Bakshi

A new simple isocratic RP-HPLC method was developed for the determination of Betahistine Hydrochloride in controlled release tablet for?ulatio?. The separatio? ?as a?hie?ed o? Kro?asil C?? ?olu?? ???? ?? ? ?.? ??, ?μ?? i? a? iso?rati? elutio? ?ode. The ?o?ile phase consisted of Buffer: Acetonitrile in the ratio of 60: 40. The column temperature was maintained at 25°C. The detection of eluent from the column was detected using UV detector at 260nm and the flow rate was maintained at 1.5 ml/min. The method was validated in terms of system suitability, specificity, accuracy, precision, linearity, filter compatibility and solution stability.

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Citations : 440

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