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Simultaneous Estimation of Flavonoidic Compounds of Punica granatum Extract by LC-ESI-MS/MS Technique


Author(s): Tarun Kumar Dasguptaa, Priscilla M. D’Melloa, Deep Bhattacharya

Pomegranate pericarp was reported to contain ingredients like, phenolic punicalagins, gallic acid, flavan-3-ol like catechin, EGCG (Epicatechin Gallocatechin),3, 4-oxo-flavonoids like quercetin, myricetin, rutin.So far no simultaneous determination of quercetin, catechin and myricetin flavonoids in pomegranate peel has been reported. In this study LC-ESI-MS/MS was used for the simultaneous detection of three flavonoids quercetin, catechin and myricetin (bio-markers) from pomegranate peel extract.Good separation was obtained using standard solution of bio-markers within 7.5 min and analysed by electro spray ionization (ESI) techniques with the gradient mobile phase system. Selective ion monitoring (SIM) afforded by tandem mass spectrometry has greater advantage of reducing interference and enhancing sensitivity over selected ion monitoring, the peaks at the retention time of 5, 6.4 and 7 min were identified as catechin, myricetin and quercetin respectively. The technique was optimized having good specificity, linearity and precision of RSD value was less than 5%. The developed method was validated and has been applied for quantitative determination of catechin, quercetin and myricetin in the extract of agro waste of Punica granatum with negative SIM (Selective ion monitoring) mode with good precision and accuracy.

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