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Author(s): Dattatray G. Sabane*, Mukesh T. Mohite, Mahesh N. Pratapwar, Rajashri S. Patil, Tejas C. Jagtap

Three simple, precise and economical methods for UV have been developed for determination of Methotrexate in bulk formulation. Method A involves measurement of UV absorbance in Zero order derivative and Method B involves first order derivative are at 302and264nm respectively. Method C deals with Area Under Curve measurement (AUC method), which involves the calculation of integrated value of absorbance with wavelength range between 297-305nm. The drug follows Beer-Lambert’s law in the concentration range of 10-50μg/ml in all three methods. Results of analysis were validated statistically and found to be satisfactory. Thus proposed method scan be successfully applied for estimation of Methotrexate in routine analytical work.

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Citations : 440

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