Author(s): Valsamma Wilson, Sugandha S. Shetye, Kawaljit Kaur, Satish N. Ambare, Jolly Jacob
A simple, precise, accurate, and specific HPTLC method was developed and validated for estimation of Caffeic acid in methanol extract of a polyherbal formulation ‘Nalpamaradi choornam’ and its four Ficus species constituents simultaneously. The method employed TLC Aluminium plate pre-coated with silica gel F254 as the stationary phase and Toluene: Ethyl Acetate: Formic acid in the ratio 5.0: 5.0: 0.6 (v/v/v) as the mobile phase. The densitometric analysis was carried out in the absorption mode at 325nm (λmax) and fluorescence mode at 366nm. The Rf value was found to be .049 ± 0.02. The above method was validated as per ICH guidelines. Hence this method can be used for routine analysis of Caffeic acid in herbal drug samples.